Link to PDF of lab report: file:///C:/Users/Cat/Downloads/labreport.pdf
Introduction: In order to determine which ingredients fight bacteria the best, each was tested as if it was going through an antibiotic sensitivity test. After Staphylococcus aureus and Escherichia coli were streaked on the plate, instead of using antibiotic discs, sterile discs were soaked in each ingredient and placed on the plate. The ingredients were selected because they all were known for potentially killing bacteria. Once the soaked discs were placed on the plate, they were incubated for 24-48 hours and examined for a zone of inhibition which is measured in millimeters.
Purpose:The purpose was to determine whether or not the ingredients fight bacteria.
Materials:
Procedure:
Data and Observations:
Table 1: The graph below shows the zone of inhibition where the bacteria stopped growing when performed with Escherichia coli.
Introduction: In order to determine which ingredients fight bacteria the best, each was tested as if it was going through an antibiotic sensitivity test. After Staphylococcus aureus and Escherichia coli were streaked on the plate, instead of using antibiotic discs, sterile discs were soaked in each ingredient and placed on the plate. The ingredients were selected because they all were known for potentially killing bacteria. Once the soaked discs were placed on the plate, they were incubated for 24-48 hours and examined for a zone of inhibition which is measured in millimeters.
Purpose:The purpose was to determine whether or not the ingredients fight bacteria.
Materials:
- Sharpie
- Ruler
- Garlic oil
- Eucalyptus
- Lavender
- Oregano
- Tea tree
- Grown staphylococcus aureus
- Grown e.coli
- Media
- 10 Petrie discs
- hole puncher
- filter paper
Procedure:
- Gather all materials, glove and work under the biological hood.
- Label a plate with the essential oil you are using. One side will have the essential and the other will have nothing.
- Pick up the lid of the plate that contains the bacteria slightly.
- Pick up a colony of the bacteria with a cotton swab.
- Quickly close the lid on the plate of bacteria.
- Use an agar plate and take the loop containing the bacteria and lawn streak, covering the whole plate in an x, y and z streak.
- Kill the bacteria on the cotton swab by placing it in a solution made of ½ bleach and ½ distilled water.
- Soak the filter paper in the substance to be tested, the first will be a control of distilled water and place the filter paper in the center of the agar plate.
- Put the lid on and secure it with tape on the sides.
- Repeat steps 1-8, however this time in step 7 soak the filter paper in oil of oregano.
- Repeat steps 1-8, however this time in step 7 soak the filter paper in garlic oil.
- Repeat steps 1-8, however this time in step 7 soak the filter paper in tea tree oil.
- Repeat steps 1-8, however this time in step 7 soak the filter paper in eucalyptus oil.
- Repeat steps 1-8, however this time in step 7 soak the filter paper in lavender oil.
- Incubate each plate overnight at 37 degrees Celsius (98.6 degrees Fahrenheit).
- Measure the zone of inhibition in mm.
Data and Observations:
Table 1: The graph below shows the zone of inhibition where the bacteria stopped growing when performed with Escherichia coli.
Ingredients |
Zone of Inhibition |
Garlic |
16mm |
Eucalyptus |
6mm |
Lavender |
7mm |
Oregano |
Killed the entire plate of E.coli |
Tea Tree |
6mm |
Table 2: The graph below shows the zone of inhibition where the bacteria stopped growing when performed with Staphylococcus aureus.